Prestained Protein Ladder Bio Rad

Proteins were transferred to nitrocellulose membrane millipore using the mini protean ii western transfer apparatus bio rad.

Prestained protein ladder bio rad.

The protein ladder is supplied in a ready to use format for direct loading onto ge. This product is no longer available. Please contact technical support for more information on replacement product. These standards can be used for fluorescent blot detection at red wavelengths 635 nm laser or red led.

Ladders contain premixed fragments of purified dna rna or protein of known sizes in terms of base pairs or molecular weights. Prestained protein ladders the biocompare ladders and markers search tool catalogs a wide variety of reagents for sizing nucleic acid and proteins in electrophoresis applications. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue orange and green stained proteins 10 to 180 kda for use as size standards in protein electrophoresis sds page and western blotting. Prestained kaleidoscope and low range protein standards bio rad were included for us02 and sb01 respectively as indicated at the left of each gel.

B pro q emerald glycoprotein analysis of us02 purified lcca. The total protein content was estimated using the bca protein assay kit pierce and equal amounts were run on denaturing sds page gels with precision prestained protein standards bio rad. The following replacement is recommended. These standards can be detected by fluorescence when excited at appropriate wavelengths.

500 μl broad range standards 7 100 209 000 mw prestained mixture of eight proteins. Bio rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including sds page ief 2 d page and western blotting. Sharper bands allow for more accurate molecular weight estimation and stronger band intensity after blot development provides visual confirmation of transfer efficiency. Precision plus protein all blue prestained protein standards.

For sds page choose from prestained and unstained natural or recombinant protein ladders for broad high and low molecular weight ranges. Free protein standards selection magnet. Bio rad s new and improved precision plus protein dual color standards are brighter for easier identification of target proteins. Precision plus protein kaleidoscope standards are a mixture of ten multicolored recombinant proteins 10 250 kd.